Production of Ligninases and Degradation of Lignin in Agitated Submerged Cultures of Phanerochaete chrysosporium.
Identifieur interne : 001049 ( Main/Exploration ); précédent : 001048; suivant : 001050Production of Ligninases and Degradation of Lignin in Agitated Submerged Cultures of Phanerochaete chrysosporium.
Auteurs : A. J Ger [États-Unis] ; S. Croan ; T K KirkSource :
- Applied and environmental microbiology [ 0099-2240 ] ; 1985.
Abstract
Research on the extracellular hemeprotein ligninases of Phanerochaete chrysosporium has been hampered by the necessity to produce them in stationary culture. This investigation examined the effects of detergents on development of ligninase activity in agitated submerged cultures. Results show that addition of Tween 80, Tween 20, or 3-[(3-colamidopropyl)dimethylammonio]1-propanesulfonate to the cultures permits development of ligninase activity comparable to that routinely obtained in stationary cultures. The detergent-amended cultures express the entire ligninolytic system, assayed as the complete oxidation of [C]lignin to CO(2). The detergent effect is evidently not merely in facilitating release of extant enzyme. Development of ligninolytic activity in the agitated cultures, as in stationary cultures, is idiophasic. Ion-exchange fast protein-liquid chromatography indicated that the heme protein profiles in agitated and stationary cultures are very similar. These findings should make it possible to scale up production of ligninolytic enzymes in stirred tank fermentors.
DOI: 10.1128/AEM.50.5.1274-1278.1985
PubMed: 16346932
PubMed Central: PMC238738
Affiliations:
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J Ger</name><affiliation wicri:level="2"><nlm:affiliation>Forest Products Laboratory, Forest Service, U. S. Department of Agriculture, Madison, Wisconsin 53705.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Forest Products Laboratory, Forest Service, U. S. Department of Agriculture, Madison</wicri:cityArea></affiliation></author><author><name sortKey="Croan, S" sort="Croan, S" uniqKey="Croan S" first="S" last="Croan">S. Croan</name></author><author><name sortKey="Kirk, T K" sort="Kirk, T K" uniqKey="Kirk T" first="T K" last="Kirk">T K Kirk</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="1985" type="published">1985</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Research on the extracellular hemeprotein ligninases of Phanerochaete chrysosporium has been hampered by the necessity to produce them in stationary culture. This investigation examined the effects of detergents on development of ligninase activity in agitated submerged cultures. Results show that addition of Tween 80, Tween 20, or 3-[(3-colamidopropyl)dimethylammonio]1-propanesulfonate to the cultures permits development of ligninase activity comparable to that routinely obtained in stationary cultures. The detergent-amended cultures express the entire ligninolytic system, assayed as the complete oxidation of [C]lignin to CO(2). The detergent effect is evidently not merely in facilitating release of extant enzyme. Development of ligninolytic activity in the agitated cultures, as in stationary cultures, is idiophasic. Ion-exchange fast protein-liquid chromatography indicated that the heme protein profiles in agitated and stationary cultures are very similar. 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Croan</name><name sortKey="Kirk, T K" sort="Kirk, T K" uniqKey="Kirk T" first="T K" last="Kirk">T K Kirk</name></noCountry><country name="États-Unis"><region name="Wisconsin"><name sortKey="J Ger, A" sort="J Ger, A" uniqKey="J Ger A" first="A" last="J Ger">A. J Ger</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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